ABSTRACT
Bone tissue engineering has emerged as a promising alternative therapy for patients who suffer bone fractures or defects caused by trauma, congenital diseases or tumours. However, the reconstruction of bone defects combined with osteoporosis remains a great challenge for clinicians and researchers. Based on our previous study, Ca-Si-based bioceramics (MSCs) showed enhanced bone formation capabilities under normal conditions, and strontium was demonstrated to be therapeutic in promoting bone quality in osteoporosis patients. Therefore, in the present study, we attempted to enlarge the application range of MSCs with Sr incorporation in an osteoporotic bone regeneration model to evaluate whether Sr could assist in regeneration outcomes. In vitro readout suggested that Sr-incorporated MSC scaffolds could enhance the expression level of osteogenic and angiogenic markers of osteoporotic bone mesenchymal stem cells (OVX BMSCs). Animal experiments showed a larger new bone area; in particular, there was a tendency for blood vessel formation to be enhanced in the Sr-MSC scaffold group, showing its positive osteogenic capacity in bone regeneration. This study systematically illustrated the effective delivery of a low-cost therapeutic Sr agent in an osteoporotic model and provided new insight into the treatment of bone defects in osteoporosis patients.
ABSTRACT
Objective: To investigate the expression of chromobox homolog 2 (CBX2) gene in colorectal cancer (CRC) tissues and cells, and to explore its clinical significance. Methods: The expressions of CBX2 mRNA and protein in CRC cells, normal colorectal epithelial cells, CRC tissues and adjacent tissues were detected by real-time fluorescent quantitative PCR and Western blotting, respectively. The expression of CBX2 in CRC tissues and their corresponding adjacent normal tissues from 66 patients was detected by immunohistochemistry. The correlations of CBX2 expression with the clinicopathological features and prognosis of patients with CRC were analyzed. Results: The expressions of CBX2 mRNA and protein in CRC cells were higher than those in normal colonic epithelial FHC cells (all P < 0.05). The expression levels of CBX2 mRNA and protein in CRC tissues were also significantly higher than those in para-cancerous tissues (all P < 0.05). The positive rate of CBX2 expression in CRC tissues was significantly higher than that in adjacent normal tissues (53.0% vs 7.6%, P < 0.05). The expression of CBX2 was associated with TNM stage, lymph node metastasis, distant metastasis, and the survival status (all P < 0.05). The overall survival time of CRC patients with high expression of CBX2 was shorter than that of CRC patients with low expression of CBX2 (P = 0.01). Both CBX2 expression and distant metastasis were independent prognostic factors in patients with CRC (both P < 0.05). Conclusion: CBX2 is overexpressed in CRC tissues and cells, and is partly involved in the occurrence and development of CRC. Moreover, the expression of CBX2 is an independent prognostic factor for the patients with CRC.
ABSTRACT
Objective To study the expression and clinical significance of ARHGAP4 in colorectal cancer Method Real?time PCR,Western blot and immunocytochemistry were used to detect the expression of ARHGAP4 in colorectal cancer tissues and cell lines ,and the correlation between its expression and clinical features of patients was analyzed Results ARHGAP4 is overexpressed in colorectal cancer tissues and cell lines and its overexpression is correlated with T stage, N stage, clinical stage, and metastasis. Conclusion ARHGAP4 may promote the progression of colorectal cancer ,and have the potential to be a novel prognosis marker.
ABSTRACT
<p><b>OBJECTIVE</b>This study was about the influence of porcelain thickness on crack at interface.</p><p><b>METHODS</b>The effect of porcelain thickness on the flaw at the interface between porcelain and metal was studied in three groups with porcelain thickness of 0.5 mm, 1.5 mm and 2.5 mm (metal thickness of 0.5 mm) by means of moire interferometre and interfacial fracture mechanics. The parameter Jc was compared among the three groups and the growing of the flaw was observed.</p><p><b>RESULTS</b>Jc and the extreme strength of group with porcelain thickness of 0.5 mm (2.813 N/m and 9.979 N) were lower than those of the groups with porcelain thickness of 1.5 mm and 2.5 mm (5.395 N/m, 19.134 N and 5.429 N/m, 19.256 N). Flaws extend along the interface in the groups with porcelain thickness of 1.5 mm and 0.5 mm.</p><p><b>CONCLUSIONS</b>(1) Fracture resistance of the interface in the groups with porcelain thickness of 1.5 mm and 2.5 mm is similar and it decreases in the group with 0.5 mm thick porcelain. (2) When porcelain is 1.5 mm or 0.5 mm thick, flaws will extend along the interface. When porcelain is 2.5 mm thick, flaws will extend into the porcelain layer.</p>